解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Aging is associated with a significant decline in secretion of growth hormone. This in turn leads to reduced circulating IGF-I and changes in IGF-binding proteins. Growth hormone replacement to growth hormone-deficient individuals has been shown to improve quality of life, enhance bone and muscle mass, and reduce card...
journal_title:Endocrine
pub_type: 杂志文章,评审
doi:10.1385/ENDO:12:2:197
更新日期:2000-04-01 00:00:00
abstract::Computer analysis of the human placental lactogen-B (hPL-B) enhancer reveals two putative binding sites for the transcription factor NF-IL6, but the role of NF-IL6 in the regulation of the enhancer is unknown. Using gel mobility shift and supershift assays, we demonstrated that NF-IL6 binds to both enhancer sites. Tra...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:12:1:47
更新日期:2000-02-01 00:00:00
abstract::This study examined the interrelationship between transforming growth factor-beta1 (TGF-beta1) and 17beta-estradiol (E2) in the regulation of growth plate chondrocytes. To determine whether TGF-beta1 modulates chondrocyte response to E2, we used cells isolated from the resting zone (RC) and growth zone (GC) of costoch...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:11:3:241
更新日期:1999-12-01 00:00:00
abstract::Steroid hormones regulate levels of gonadotropin mRNA in the pituitary, and gonadotropic hormones in plasma. To determine whether estrogen receptor alpha (ERalpha) mediates steroid negative feedback, wild type (WT) and estrogen receptor alpha knockout (ERalphaKO) mice of both sexes were gonadectomized and implanted wi...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:11:2:137
更新日期:1999-10-01 00:00:00
abstract::Factors affecting longevity are complex and poorly understood. We have recently found that Ames dwarf mice (df/df), which are deficient in growth hormone (GH), prolactin, and thyroid-stimulating hormone, live significantly longer than their normal siblings whereas transgenic mice that overexpress GH exhibit reduced li...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:11:1:41
更新日期:1999-08-01 00:00:00
abstract::We have linked two human chorionic gonadotropin (hCG) beta-subunit cDNAs in tandem such that the expressed fusion protein consists of two mature beta-subunits joined through the carboxy terminal peptide of the first beta-subunit. A single glycine residue is inserted between the two subunits in the fusion protein. Chin...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738625
更新日期:1999-06-01 00:00:00
abstract::Mammary cell mitogenic responsiveness was evaluated with an established nontransformed caprine mammary epithelial cell line (CMEC). As expected, the cells responded to insulin-like growth factor 1 (IGF-1), insulin, and hydrocortisone with an increased number of cells after 3 and 5 d in culture. In combination, insulin...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:10:2:123
更新日期:1999-04-01 00:00:00
abstract::Detection of connexin (Cx) proteins has been used as an indicator of the presence of structural and functional gap junctions in tissues. To examine the role of gap junctions during follicular growth and atresia, the presence of three major connexins, Cx43, Cx32, and Cx26, was evaluated in bovine ovaries by using immun...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:10:1:43
更新日期:1999-02-01 00:00:00
abstract::The androgen receptor (AR) plays a critical role in sexual differentiation and in the virilization of the male reproductive system. A clear understanding of AR expression at the early stages of sexual development will help elucidate the sensitivity of perinatal animals to endocrine modulation by external agents, such ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:9:3:253
更新日期:1998-12-01 00:00:00
abstract::Cholecystokinin (CCK) amide concentrations were reduced over 85% in all the major brain regions of carboxypeptidase E (Cpe)(fat)/Cpe(fat) mice in comparison to control mice. Using an radioimmunoassay (RIA) specific for glycine-extended CCK (CCK Gly), low levels of CCK Gly were detected in control (0.65 ng/g tissue) an...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:9:3:329
更新日期:1998-12-01 00:00:00
abstract::Lactogen-dependent Nb2 lymphoma cells, widely employed for studying prolactin (PRL) mitogenic mechanisms, are also useful for investigations of apoptosis in T-lineage lymphocytes. Utilizing PRL-dependent Nb2-11 cultures, apoptosis-regulatory genes were evaluated for participation in dexamethasone- (DEX) provoked cell ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:9:2:163
更新日期:1998-10-01 00:00:00
abstract::In these studies, we show that endothelin (ET), leukemia inhibitory factor (LIF), phenylephrine (PE), and prostaglandin F2alpha (PGF2alpha), which are all hypertrophic for neonatal rat cardiac myocytes in culture, induce distinct morphological, physiological, and genetic changes after a 48-h treatment. Transmission el...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/endo:9:1:45
更新日期:1998-08-01 00:00:00
abstract::To determine whether murine mammary growth is modulated by local insulin-like growth factor-1 (IGF-1) production, expression of recombinant IGF-1 was directed to the mammary glands of transgenic mice using an ovine prepro IGF-1 cDNA under control of the mouse mammary tumor virus-long terminal repeat (MMTV-LTR) promote...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:8:3:251
更新日期:1998-06-01 00:00:00
abstract::Glucocorticoid hormones potentiate the toxic effects of neuronal stressors. Alteration of gene expression by glucocorticoids could contribute to neuronal susceptibility by downregulating the synthesis of proteins necessary to adapt to challenge. Using heat shock of hippocampal slices as a model for cellular insult, pr...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:8:2:135
更新日期:1998-04-01 00:00:00
abstract::Hyponatremia has been observed in elderly patients treated with the selective serotonin reuptake inhibitor (SSRI) fluoxetine. The pathogenesis of this effect is not known, but enhanced release of vasopressin (VP) and its renal actions may be a possible mechanism. Excess secretion of VP in combination with large fluid ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:8:1:13
更新日期:1998-02-01 00:00:00
abstract::Leptin is an important hormone that has potent effects on appetite and body weight. The regulation of leptin gene expression and secretion by corticosteroids and insulin was studied in the rat. Adrenalectomy resulted in a significant reduction in leptin gene expression and secretion. The reduction was corrected by hor...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1385/ENDO:8:1:85
更新日期:1998-02-01 00:00:00
abstract::Patients with ectopic ACTH syndrome often develop hypertension and hypokalemic alkalosis with an abnormal increase in the ratio of plasma cortisol to cortisone, indicating that 11 beta-hydroxysteroid dehydrogenase (11 beta HSD) activity is inhibited. Inhibition of 11 beta HSD allows access of cortisol or corticosteron...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02801327
更新日期:1997-12-01 00:00:00
abstract::Succinic acid esters are currently under investigation as possible insulinotropic tools in the treatment of noninsulin-dependent diabetes mellitus. The present article introduces three novel nutrient esters and aims mainly to explore, in both normal and GK rats, the secretory response to such esters when tested alone ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02778136
更新日期:1997-10-01 00:00:00
abstract::Interactions between testosterone, estradiol, and inhibin in the control of gonadotrophin secretion in males are poorly understood. Castrated rams were treated with steroid-free bovine follicular fluid (bFF), testosterone, or estradiol and for 7 d (2 x 2 x 2 factorial design). Given independently, none of the exogenou...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02778146
更新日期:1997-10-01 00:00:00
abstract::Remarkable decreases in growth hormone (GH) secretion accompany healthy aging. The pathophysiology of this hyposomatotropism is confounded by concurrent changes in body composition (with increased visceral fat), physiological declines in estrogen and androgen concentrations, differences in gender responses to aging, a...
journal_title:Endocrine
pub_type: 杂志文章,评审
doi:10.1007/BF02778061
更新日期:1997-08-01 00:00:00
abstract::Steroidogenic factor-1 (SF-1) is a transcription factor involved in regulation of steroidogenic enzymes. Recent evidence indicates that SF-1 is also important in the anterior pituitary gland, where it may influence gene expression in gonadotropes. We isolated a cDNA encoding ovine SF-1 and demonstrated that the SF-1 g...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02820500
更新日期:1997-06-01 00:00:00
abstract::Stimulation of dopamine D2 receptors inhibits melanotrope pro-opiomelanocortin (POMC) biosynthesis and alpha-melanocyte-stimulating hormone (MSH) secretion. These effects are mediated by G-protein alpha i- and alpha o-subunits and are reversed by stimulating receptors linked to activation of G alpha s protein. Melanot...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02820510
更新日期:1997-06-01 00:00:00
abstract::The effect of stress on serum corticosteroid-binding globulin (CBG) was studied in adult male Sprague-Dawley rats. CBG was measured either by a homologous radioimmunoassay (RIA) or by a binding assay (BA) using 3H-corticosterone. Exposure of adult male rats to a severe stressor such as immobilization (IMO) for 1 h did...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738959
更新日期:1997-04-01 00:00:00
abstract::Plasma levels for insulin-like growth factor-I (IGF-I) steadily increase in female rats between 20 and 40 d of life, and this increase is intimately related to the wellknown growth spurt occurring at this age. Since specific actions of IGF-I related to sexual function have been described at the ovarian and hypothalami...
journal_title:Endocrine
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::The cytoplasmic Ca2+ concentration ([Ca2+]i) was measured with dual wavelength fluorometry in glucagon-producing mouse pancreatic alpha-cells loaded with the indicator fura-2. Spontaneous rhythmic activity in terms of slow oscillations from a basal level was observed at 3 mM glucose. Like in the insulin-secreting beta...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738806
更新日期:1997-02-01 00:00:00
abstract::Interleukin-6 (IL-6) and tumor necrosis factor (TNF) are secreted by rat adrenal zona glomerulosa cells. Serotonin increases the release of aldosterone, corti-costerone, and cortisol from the adrenal cortex. Therefore, the effects of serotonin on IL-6 and TNF release from rat adrenal zona glomerulosa cells were invest...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02739062
更新日期:1996-12-01 00:00:00
abstract::Steroid biosynthesis begins with the enzymatic conversion of cholesterol to pregnenolone. This reaction is catalyzed by the cytochrome P450 side-chain cleavage enzyme (P450scc), which is located on the matrix side of the inner mitochondrial membrane. Although the rate-limiting enzymatic step in steroidogenesis is the ...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738705
更新日期:1996-10-01 00:00:00
abstract::In arthropods, development is controlled by cholesterol-derived steroid hormones: the ecdysteroids. In vertebrates and insects, steroidogenesis is positively regulated and this is mediated by cAMP. In crustaceans, ecdysteroid biosynthesis by steroidogenic organs (Y-organs) is negatively regulated by a neuropeptide, th...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738652
更新日期:1996-08-01 00:00:00
abstract::Mammalian reproduction is dependent on both a successful pregnancy and on the subsequent period of lactation. In the rat, ovulation occurs shortly after parturition making it possible for a dam to be simultaneously pregnant and lactating. The present studies investigate the effect of placental hormones on suckling-ind...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738662
更新日期:1996-08-01 00:00:00
abstract::Cytoplasmic calcium ([Ca(2+)](i)) changes evoked by adenosine 5(1)-triphosphate (ATP) were recorded in cultured individual Leydig cells within 10-18 h after cell dispersion. [Ca(2+)](i) was monitored using Fura-2AM loaded cells with a digital ratio imaging system. Five micromolars ATP induced biphasic [Ca(2+)](i) resp...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738690
更新日期:1996-06-01 00:00:00
abstract::Gonadotropin-releasing hormone (GnRH) effects on the lactotroph function have been widely studied, but they probably result from paracrine interactions. No visual data about GnRH receptor in the pituitary are available. In order to identify the GnRH target cells in the pituitary of adult rats, the cellular distributio...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02782760
更新日期:1996-04-01 00:00:00
abstract::The role of membrane-associated tubulin in TRH receptor-G protein coupling was investigated with the use of compounds that influence tubulin function. TRH-stimulated G protein GTPase activity in GH(3) cell membranes was used to determine receptor-G protein coupling. TRH-stimulated GTPase activity was abolished by G(qα...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738873
更新日期:1996-02-01 00:00:00
abstract::Oestradiol (E(2)) alters lymphocyte functionin vitro including T cell DNA synthesis and B cell immunoglobulin production in human tonsillar, splenic and peripheral blood cells. We have investigated whether one mechanism for this effect is that E(2) modifies the expression of IL-2, IL-6 and IFN-γ mRNA in human tonsilla...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02935680
更新日期:1995-11-01 00:00:00
abstract::The anterior pituitary gland of the Siberian hamster contains high concentrations of tachykinins compared to other laboratory rodents. In this investigation we studied the relative quantities of neurokinin A (NKA), neuropeptide gamma (NPG), and neuropeptide K (NPK) present in extracts of anterior pituitaries from this...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02935690
更新日期:1995-11-01 00:00:00
abstract::We have investigated the effects of different patterns of administration of recombinant human growth hormone (rhGH) on weight gain, organ growth, serum GH binding protein (GHBP) and insulin-like growth factor-l (IGF-1) levels in a series of studies using hypophysectomized (Hx) or GH-deficient dwarf (dw/dw) rats. Anima...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF03000203
更新日期:1995-10-01 00:00:00
abstract::The A1 adenosine receptor (A1AR) exerts important biological effects in the mammalian biology. To provide insights into the role A1AR action in human physiology, we characterized the pharmacologic properties of the human A1AR, examined somatic sites of A1AR gene expression, and identified the chromosomal location of t...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02746338
更新日期:1995-09-01 00:00:00
abstract::To explore the regulation of follistatin gene expression, porcine granulosa cells were incubated with the translational inhibitor, cycloheximide (CHX), for periods from 6-24 h. This resulted in a 3 to 10-fold increase in follistatin mRNA accumulation compared to vehicle treated control cultures. At 20 h, CHX augmented...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02953027
更新日期:1995-08-01 00:00:00
abstract::Melatonin, a pineal hormone, is known to be an important neurohormonal factor involved in the timing of reproductive events which occur seasonally in various mammalian species. In order to evaluate the influence of melatonin on neurons which are producing gonadotropin-releasing hormone (GnRH), we studied the effect of...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02738829
更新日期:1995-07-01 00:00:00
abstract::In order to analyse whether the participation of the dopaminergic system of the preoptic-anterior hypothalamic area (POA-AHA) in the regulation of ovulation is asymmetric and varies during the estrous cycle of the rat, as it occurs with the cholinergic system, the effects of a unilateral implant of haloperidol were st...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02935642
更新日期:1995-06-01 00:00:00
abstract::Estrogens increase prolactin (PRL) synthesis and release in rats and humans, whereas pituitary-derived calcitonin-like immunoreactive peptide (pit-CT) inhibits PRL gene expression and release. To test the hypothesis that estrogens stimulate lactotrophs by diminishing pit-CT expression, the present studies examined eff...
journal_title:Endocrine
pub_type: 杂志文章
doi:10.1007/BF02935652
更新日期:1995-06-01 00:00:00